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Updated: May 20, 2026

A Behavioral Assay for Investigating the Role of Spatial Memory During Instinctive Defense in Mice
Published on: July 21, 2018
Mice recognize the center of an enclosure
Naomi Inoue1, Shigeru Watanabe
1Department of Psychology, Keio University, 2-2-24 Mita, Minato-ku, Tokyo, 108-8345, Japan.
Abstract:
In recent years, it has been shown that animals can localize the geometric center of an area by reference to the shape of the environment. We trained a group of mice (experimental group) to search for a pellet hidden under sand in the center of a square-shaped dry maze. Three weeks later, they were tested in a triangular enclosure half the size of the training area and a circular enclosure double the size of the training area to see transfer to these enclosures. We compared their searching behavior with that of subjects that had received no training. The results show that the experimental group searched the geometric center of each enclosure in both transfer tests, while the untrained control group walked along the walls. This indicates that the experimental group localized the center not by reference to the absolute distance from the corners but by equal distances from all walls (geometric center).

