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Updated: May 20, 2026
Characterization of Molecular Mechanisms of In vivo UVR Induced Cataract
Published on: November 28, 2012
[Effect of ultraviolet radiation on ALDH1 expression in human lens epithelial cells]
Jingming Shi1, Songbai Jia, Xuan Chen
1Department of Ophthalmology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Objective:
To determine the apoptosis-inducing effect of ultraviolet light (UV) on human lens epithelial cell (HLEC) and to explore the involvement of changes in ALDH1 folowing UV radiation.
Methods:
HLEC was exposed to the same UV light source and was subsequently divided into 6 groups according to UV radiation time of 0 (control group), 5, 10, 15, and 30 min. Apoptosis was detected by AO/EB staining. Changes of ALDH1 in HLEC were detected by immunohistochemical staining and Western blot.
Results:
The intensity of immunohistochemical staining and the rate of positive cells decreased with increase of UV time (P<0.05). The rate of positive ALDH1 cells was negatively correlated with the rate of apoptosis (r= -0.92, P<0.05). Western blot showed the integrated absorbance values significantly decreased with the increase of UV time (P<0.05).
Conclusion:
ALDH1 in HLEC decreases with an increase of UV exposure, which may be related to UV induced apoptosis of HLEC.
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