Related Experiment Video
Updated: May 20, 2026
Isolation and Time-Lapse Imaging of Primary Mouse Embryonic Palatal Mesenchyme Cells to Analyze Collective Movement Attributes
Published on: February 13, 2021
TLM-Tracker: software for cell segmentation, tracking and lineage analysis in time-lapse microscopy movies
Johannes Klein1, Stefan Leupold, Ilona Biegler
1Institute of Microbiology, Technische Universität Braunschweig, Braunschweig, Germany.
Motivation:
Time-lapse imaging in combination with fluorescence microscopy techniques enable the investigation of gene regulatory circuits and uncovered phenomena like culture heterogeneity. In this context, computational image processing for the analysis of single cell behaviour plays an increasing role in systems biology and mathematical modelling approaches. Consequently, we developed a software package with graphical user interface for the analysis of single bacterial cell behaviour.
Results:
A new software called TLM-Tracker allows for the flexible and user-friendly interpretation for the segmentation, tracking and lineage analysis of microbial cells in time-lapse movies.
Availability:
The software package, including manual, tutorial video and examples, is available as Matlab code or executable binaries at http://www.tlmtracker.tu-bs.de.
