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Inhibitory receptor expression on neonatal immune cells
J Walk1, G H A Westerlaken, N O van Uden
1Department of Paediatrics, Wilhelmina Children's Hospital, Utrecht, The Netherlands.
Clinical and Experimental Immunology
|July 11, 2012
Summary
Neonatal immune cells show increased expression of inhibitory receptors, impacting their function. This study reveals distinct inhibitory receptor patterns in newborns, potentially preventing allergies and autoimmunity.
Area of Science:
- Immunology
- Neonatal Health
- Cell Biology
Background:
- Neonates exhibit immune system defects in both adaptive and innate responses.
- Inhibitory receptor signaling regulates immune cell activation thresholds and function.
- Increased inhibitory receptor expression may suppress neonatal immune cell activity.
Purpose of the Study:
- To investigate if seven specific inhibitory receptors are upregulated on neonatal immune cells compared to adult cells.
- To characterize the expression patterns of inhibitory receptors on various neonatal immune cell types.
Main Methods:
- Flow cytometry was used to analyze the expression of seven inhibitory receptors (LAIR-1, SIRL-1, CD31, SIRPα, Siglec-9, CD200R, IREM-1) and CD200.
- Leukocytes were analyzed from cord blood, neonatal venous blood, and adult venous blood samples.
- Immune cell subsets examined included neutrophils, monocytes, NK cells, CD4(+) T cells, and CD8(+) T cells.
Main Results:
- Expression of leucocyte-associated immunoglobulin (Ig)-like receptor-1 (LAIR-1), CD31, and CD200 was consistently increased on all neonatal T cell subsets.
- Neonatal monocytes showed decreased expression of LAIR-1 and immune receptor expressed on myeloid cells-1 (IREM-1) compared to adults.
- A unique LAIR-1-positive neutrophil population was identified in cord and neonatal blood, absent in adults.
Conclusions:
- Distinct inhibitory receptor expression profiles exist on neonatal peripheral blood immune cells.
- This study provides the first evidence of inhibitory receptors regulating the neonatal immune system.
- Elevated inhibitory receptor expression on neonatal T cells may play a role in preventing allergy and autoimmunity.
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