Related Experiment Video

Updated: May 20, 2026

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus
13:41

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

Published on: March 8, 2012

NF-κB signalling is attenuated by the E7 protein from cutaneous human papillomaviruses

Luise M Byg1, Jessica Vidlund, Natasa Vasiljevic

  • 1The DNA Tumour Virus Group, Department of Cellular and Molecular Medicine, University of Copenhagen, Denmark.

Virus Research
|July 11, 2012
PubMed

Insights

Cutaneous human papillomaviruses (HPVs) E7 proteins inhibit the NF-κB pathway, similar to high-risk Alpha-HPV types. This suggests a role for Beta- and Gamma-HPV E7 in non-melanoma skin cancer development.

Area of Science:

  • Oncology
  • Virology
  • Molecular Biology

Background:

  • High-risk Alpha-papillomaviruses (HPVs) cause cervical cancer and their E7 proteins modulate the NF-κB pathway.
  • Cutaneous Beta-papillomaviruses are linked to non-melanoma skin cancer, but their effect on the NF-κB pathway is unknown.

Purpose of the Study:

  • To investigate whether E7 proteins from cutaneous Beta- and Gamma-papillomaviruses can modulate the NF-κB pathway.
  • To determine if these E7 proteins interact with IKKα and IKKβ.

Main Methods:

  • E7 proteins from seven cutaneous HPV types (Beta genus: HPV-20, -37, -38, -92, -93, -96; Gamma genus: HPV-4) were expressed in U2OS cells.
  • NF-κB pathway activity was assessed.
  • Interactions with IKKα and IKKβ were examined.

Main Results:

  • E7 proteins from all tested cutaneous HPV types inhibited NF-κB activity.
  • Cutaneous HPV E7 proteins interacted with IKKα but not IKKβ.
  • This inhibitory effect mirrors that of high-risk Alpha-HPV16 E7.

Conclusions:

  • E7 proteins from Beta- and Gamma-papillomaviruses can inhibit the NF-κB pathway.
  • This inhibition, mediated by IKKα interaction, may contribute to the pathogenesis of non-melanoma skin cancers.

More Related Videos

In situ Subcellular Fractionation of Adherent and Non-adherent Mammalian Cells09:20

In situ Subcellular Fractionation of Adherent and Non-adherent Mammalian Cells

Published on: July 23, 2010

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production08:32

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production

Published on: March 2, 2014

Related Concept Videos

NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
NF-kB-dependent Signaling Pathway02:26

NF-kB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR activation may...
MAPK Signaling Cascades01:07

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
Nucleotide Excision Repair01:38

Nucleotide Excision Repair

DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...