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Updated: May 20, 2026

Hippocampal Insulin Microinjection and In vivo Microdialysis During Spatial Memory Testing

Hippocampal Insulin Microinjection and In vivo Microdialysis During Spatial Memory Testing

Published on: January 11, 2013

Insulin and memory in Lymnaea.

E Ito¹, R Okada, Yuki Sakamoto

¹Laboratory of Functional Biology, Kagawa School of Pharmaceutical Sciences, Tokushima Bunri University, Sanuki, Japan. eito@kph.bunri-u.ac.jp

Acta Biologica Hungarica

|July 11, 2012

Summary

This summary is machine-generated.

Molluscan insulin-related peptides (MIPs) are involved in long-term memory (LTM) consolidation in pond snails. Blocking MIP receptors with an antibody inhibited LTM formation but not initial learning.

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Area of Science:

Neuroscience
Memory Research
Cellular Biology

Background:

Pond snails (Lymnaea stagnalis) exhibit conditioned taste aversion (CTA) and long-term memory (LTM).
Molluscan insulin-related peptides (MIPs) are upregulated during CTA-LTM.
MIPs may play a role in synaptic plasticity underlying memory consolidation.

Purpose of the Study:

To investigate the role of MIPs and their receptors in CTA-LTM consolidation.
To test the hypothesis that MIP receptors are crucial for memory consolidation at specific synapses.

Main Methods:

DNA microarray analysis to identify upregulated MIPs.
Electrophysiology to assess synaptic plasticity (long-term potentiation, LTP).
Application of an antibody against insulin receptors (presumed MIP receptor cross-reactivity) to the snail central nervous system (CNS) and whole animals.

Main Results:

Application of the antibody to isolated CNS blocked LTP.
Injection of the antibody into snails inhibited LTM consolidation.
The antibody did not affect the initial formation of CTA.

Conclusions:

MIP receptors are critical for the consolidation of long-term memory in Lymnaea stagnalis.
These receptors likely act at synapses involved in memory formation, specifically during the consolidation phase.
The findings highlight a conserved role for insulin signaling pathways in memory processes across species.