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Expression patterns of five polymorphic membrane proteins during the Chlamydia abortus developmental cycle.

Nick Wheelhouse¹, Michelle Sait, Kim Wilson

¹Moredun Research Institute, Pentlands Science Park, Bush Loan, Edinburgh, Midlothian EH26 0PZ, UK. nick.wheelhouse@moredun.ac.uk

Veterinary Microbiology

|July 11, 2012

Summary

This summary is machine-generated.

Polymorphic membrane proteins (Pmps) in Chlamydia abortus show varied expression patterns during infection. Some Pmps are present throughout the cycle, while others appear later, suggesting diverse roles and potential antigenic variation.

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Area of Science:

Microbiology
Bacterial Pathogenesis
Immunology

Background:

Polymorphic membrane proteins (Pmps) are implicated in Chlamydia abortus pathogenesis.
Previous work confirmed Pmp gene expression at the transcriptional level.

Purpose of the Study:

To quantify Pmp-positive inclusions during the C. abortus developmental cycle.
To investigate heterogeneity in Pmp expression patterns.

Main Methods:

Infection of McCoy cells with C. abortus.
Analysis of Pmp expression over 72 hours using fluorescent immunocytochemistry.

Main Results:

Pmp18D was detected throughout the 72-hour cycle, quantifiable from 36 hours post-infection (hpi).
Pmp10G inclusions were visible from 36 hpi.
Pmps 13G, 16G, and 17G were detected later in the cycle in less than half of inclusions.

Conclusions:

Pmp expression in C. abortus is heterogeneous; some Pmps are constitutive (e.g., Pmp18D), others vary.
Different Pmps likely have distinct roles throughout the C. abortus developmental cycle.
Variable Pmp expression may contribute to antigenic variation.