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Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

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Genetically targetable and color-switching fluorescent probe.

Dmytro A Yushchenko¹, Ming Zhang, Qi Yan

¹Molecular Biosensor and Imaging Center, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA 15213, USA. ydmytry@gmail.com

Chembiochem : a European Journal of Chemical Biology

|July 11, 2012

Summary

This summary is machine-generated.

Researchers created a novel probe, TMR-para-MG, that changes color when binding to a fluorogen-activating protein (FAP). This dye can label cellular targets and mitochondria distinctly, aiding in monitoring mitochondrial membrane potential.

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Area of Science:

Biochemistry
Cell Biology
Molecular Probes

Background:

Mitochondrial membrane potential is crucial for cellular function.
Existing probes for mitochondrial potential have limitations.

Purpose of the Study:

To develop a novel fluorescent probe for cellular imaging.
To assess the utility of the probe for monitoring mitochondrial membrane potential.

Main Methods:

Development of the TMR-para-MG fluorescent probe.
Utilizing fluorogen-activating protein (FAP) technology for targeted labeling.
Cellular experiments to test probe performance in FAP-expressing cells.

Main Results:

The TMR-para-MG probe exhibits fluorescence switching upon binding to FAP.
The dye successfully labels target sites in one color and mitochondria in another.
Demonstrated potential for monitoring changes in mitochondrial membrane potential.

Conclusions:

TMR-para-MG is a versatile fluorescent probe for dual-color cell labeling.
The probe shows promise as a tool for assessing mitochondrial membrane potential.