An electrochemical DNA microbiosensor based on succinimide-modified acrylic microspheres

Area of Science:

• Biotechnology
• Electrochemistry
• Materials Science

Background:

• Development of sensitive and reliable DNA detection methods is crucial for diagnostics.
• Acrylic microspheres offer a versatile platform for biosensor fabrication.
• N-acryloxysuccinimide (NAS) functional groups enable efficient biomolecule immobilization.

Purpose of the Study:

• To fabricate an electrochemical microbiosensor for DNA detection.
• To utilize NAS-functionalized acrylic microspheres for DNA probe immobilization.
• To evaluate the analytical performance and optimize the conditions for the developed DNA biosensor.

Main Methods:

• Synthesis of hydrophobic poly(n-butylacrylate-N-acryloxysuccinimide) microspheres via photopolymerization.
• Immobilization of aminated DNA probes onto NAS-modified microspheres through covalent bonding.
• Hybridization detection using differential pulse voltammetry with anthraquninone-2-sulfonic acid monohydrate sodium salt (AQMS) as a label.

Main Results:

• The DNA microbiosensor exhibited a linear response over a wide concentration range (1.0 × 10⁻¹⁶ to 1.0 × 10⁻⁸ M).
• A low limit of detection (LOD) of 9.46 × 10⁻¹⁷ M was achieved with good linearity (R² = 0.97).
• The biosensor demonstrated good reproducibility with a relative standard deviation of 2.84% (n=3).

Conclusions:

• NAS-modified acrylic microspheres provide an effective matrix for DNA immobilization in electrochemical biosensors.
• The developed DNA microbiosensor shows enhanced analytical performance compared to other nano-materials.
• This approach offers a promising platform for sensitive and reliable DNA detection.