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Updated: May 20, 2026
Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes
Published on: September 26, 2013
[Modulatory effect of triptolide on differentiation of human Th17 cells]
Pengyuan Chen1, Rui Han, Qiang Zhou
1Department of Dermatology, Sir Run Run Shaw Hospital, Medical School, Zhejiang University, Hangzhou 310016, China.
Objective:
To investigate the effect of triptolide on the differentiation of human Th17 cells.
Method:
Human peripheral blood mononuclear cells, purified CD4+ T cells and CD4+CD45RA- memory T cells were treated with various concentrations of triptolide in vitro. Cell proliferation was determined by MTT assay. Flow cytometry was used to analyze the intracellular expression of IL-17 and IFN-gamma. Cytokine production of IL-17 and IFN-gamma was measured by ELISA.
Result:
Cell proliferation, intracellular expression of IL-17 and IL-17 secretion were inhibited by triptolide in a dose-dependent manner. IFN-gamma expression and production were also inhibited by triptolide.
Conclusion:
Triptolide inhibits the differentiation of human Th17 cell. The observation may indicate at least one of the mechanisms of the immunosuppressive and anti-inflammatory effects of triptolide.
