[Comprehensive chemical pattern recognition of pantagirus semen]

Area of Science:

• Phytochemistry
• Chemometrics
• Analytical Chemistry

Context:

• Plantaginis Semen (plantain seed) is a traditional herbal medicine with varying chemical profiles.
• Standardization of herbal medicines is crucial for ensuring efficacy and safety.
• Chemical fingerprinting is a powerful tool for characterizing complex natural products.

Purpose:

• To establish a robust method for comprehensive chemical pattern recognition of Plantaginis Semen.
• To utilize High-Performance Liquid Chromatography (HPLC) fingerprinting combined with chemometric methods (PCA and cluster analysis).
• To develop a reliable approach for quality control and evaluation of Plantaginis Semen.

Summary:

• HPLC fingerprinting was performed on 24 batches of Plantaginis Semen using a C18 column and a gradient elution mobile phase.
• Ten common peaks were identified in the HPLC fingerprints of the ethyl acetate fraction.
• Principal Component Analysis (PCA) and cluster analysis revealed similar but distinct patterns, highlighting method efficacy.

Impact:

• The developed method provides a comprehensive approach for chemical pattern recognition of Plantaginis Semen.
• This methodology can be applied for effective quality control and standardization of this important medicinal plant.
• The study contributes to the reliable evaluation and utilization of herbal medicines based on their chemical composition.