Related Experiment Video
Updated: May 20, 2026

Multi-analyte Biochip (MAB) Based on All-solid-state Ion-selective Electrodes (ASSISE) for Physiological Research
Published on: April 18, 2013
Novel creatine biosensors based on all solid-state contact ammonium-selective membrane electrodes
Melda Altikatoglu1, Emine Karakus, Vildan Erci
1Yildiz Technical University, Faculty of Arts and Sciences, Department of Chemistry, Davutpasa Campus, Esenler, 34210 Istanbul, Turkey.
Abstract:
Novel creatine bienzymatic potentiometric biosensors were prepared by immobilizing urease and creatinase on all solid-state contact PVC-containing palmitic acid and carboxylated PVC matrix membrane ammonium-selective electrodes without inner reference solution. Potentiometric characteristics of biosensors were examined in physiological model solutions at different creatine concentrations. The linear working range and long-term sensitivity of the biosensors were also determined. The creatine biosensors prepared by using the carboxylated PVC membrane electrodes showed more effective performance than those of the PVC containing palmitic acid membrane electrodes. Creatine assay in serum samples was successfully carried out by using the standard addition method.
Related Concept Videos
Potentiometry: Membrane Electrodes
Microbial Biosensors
Amperometry: Overview

