Novel creatine biosensors based on all solid-state contact ammonium-selective membrane electrodes.

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Electrochemistry

Background:

• Biosensors offer sensitive detection methods for biological analytes.
• Potentiometric biosensors provide a non-invasive and cost-effective analytical tool.
• Creatine measurement is crucial for diagnosing various metabolic disorders.

Purpose of the Study:

• To develop novel, all-solid-state potentiometric biosensors for creatine detection.
• To compare the performance of biosensors utilizing different PVC membrane compositions.
• To evaluate the applicability of the developed biosensors for creatine analysis in biological samples.

Main Methods:

• Immobilization of urease and creatinase enzymes onto solid-state contact PVC-based electrodes.
• Preparation of electrodes with palmitic acid and carboxylated PVC membranes.
• Potentiometric characterization in physiological model solutions and serum samples.
• Determination of linear working range and long-term sensitivity.

Main Results:

• Successfully developed novel bienzymatic potentiometric biosensors for creatine.
• Carboxylated PVC membrane electrodes demonstrated superior performance over palmitic acid membranes.
• Biosensors exhibited a defined linear working range and stable long-term sensitivity.
• Accurate creatine quantification in serum samples was achieved using the standard addition method.

Conclusions:

• Novel all-solid-state potentiometric biosensors for creatine detection were successfully developed.
• Carboxylated PVC matrix membrane electrodes are more effective for creatine biosensing.
• The developed biosensors show promise for routine creatine analysis in clinical settings.