Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Viruses with RNA Genomes01:29

Viruses with RNA Genomes

RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identification of cellular intermediates unveils unique enzymes for flagellar glycan biosynthesis in <i>Clostridioides difficile</i>.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Synthesis and Immunological Evaluation of Pel-Derived Glycoconjugate Vaccine Candidates against <i>Pseudomonas aeruginosa</i>.

JACS Au·2026
Same author

Specificity and recognition of the ADP-ribosyl-ubiquitin modification in the DNA damage response.

PLoS biology·2026
Same author

Oseltamivir aziridines are potent influenza neuraminidase inhibitors and imaging agents.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Fully protected pyrophosphates <i>via</i> phosphorobromidates for the synthesis of biopolymers.

Chemical science·2026
Same author

Histidine and tyrosine residues are targets for SIRT6 ADP-ribosylation activity.

Open biology·2026

Related Experiment Video

Updated: May 20, 2026

Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus
09:08

Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus

Published on: July 27, 2021

Fully automated sequential solid phase approach towards viral RNA-nucleopeptides.

Gerbrand J van der Heden van Noort1, Pieter van Delft, Nico J Meeuwenoord

  • 1Leiden Institute of Chemistry, Leiden University, P. O. Box 9502, 2300 RA Leiden, The Netherlands.

Chemical Communications (Cambridge, England)
|July 12, 2012
PubMed
Summary

Researchers synthesized complex ribonucleoprotein fragments using automated peptide and oligonucleotide synthesizers. This novel method combines both syntheses on a single solid support for hybrid biomolecule preparation.

More Related Videos

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism
11:37

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

Published on: July 28, 2017

Unbiased Deep Sequencing of RNA Viruses from Clinical Samples
09:36

Unbiased Deep Sequencing of RNA Viruses from Clinical Samples

Published on: July 2, 2016

Related Experiment Videos

Last Updated: May 20, 2026

Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus
09:08

Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus

Published on: July 27, 2021

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism
11:37

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

Published on: July 28, 2017

Unbiased Deep Sequencing of RNA Viruses from Clinical Samples
09:36

Unbiased Deep Sequencing of RNA Viruses from Clinical Samples

Published on: July 2, 2016

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Chemical Synthesis

Background:

  • Ribonucleoproteins (RNPs) are crucial for various cellular processes.
  • Synthesizing complex RNP fragments poses significant challenges.
  • Existing methods often lack efficiency and versatility.

Purpose of the Study:

  • To report the synthesis of two novel ribonucleoprotein fragments.
  • To demonstrate a new method for preparing complex hybrid biomolecules.
  • To advance the field of biomolecular synthesis.

Main Methods:

  • Utilized an automated solid-phase peptide synthesizer.
  • Employed an automated solid-phase oligonucleotide synthesizer.
  • Combined both synthesizers on a single solid support.

Main Results:

  • Successfully synthesized two ribonucleoprotein fragments of unprecedented complexity.
  • Demonstrated the feasibility of a combined automated synthesis approach.
  • Produced hybrid biomolecules with potential for diverse applications.

Conclusions:

  • The combined automated synthesis approach is effective for creating complex RNP fragments.
  • This method offers a novel pathway for hybrid biomolecule production.
  • The synthesized fragments hold promise for future research in molecular biology and drug discovery.