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Affinity Chromatography

Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...

Types Of Column Chromatography

Types Of Column Chromatography

The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
When the...

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Related Experiment Video

Updated: May 20, 2026

Detection of Protein S-Acylation using Acyl-Resin Assisted Capture

Detection of Protein S-Acylation using Acyl-Resin Assisted Capture

Published on: April 10, 2020

[Detecting acetylated proteins by affinity chromatography column].

Li Zheng¹, Yan-ping Zhong, Hao Xiao

¹Guangxi Medical University, Nanning, China.

Zhonghua Xue Ye Xue Za Zhi = Zhonghua Xueyexue Zazhi

|July 12, 2012

Summary

This study presents a new method for detecting acetylated proteins using affinity chromatography. The technique successfully identified multiple acetylated proteins, including histones, and can screen agents targeting histone deacetylase.

Area of Science:

Biochemistry
Proteomics
Cell Biology

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Related Experiment Videos

Last Updated: May 20, 2026

Detection of Protein S-Acylation using Acyl-Resin Assisted Capture

Detection of Protein S-Acylation using Acyl-Resin Assisted Capture

Published on: April 10, 2020

A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli

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Published on: December 9, 2017

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020