[Continuous intracorporeal glucose measurement using enzyme electrodes].

Area of Science:

• Biomedical Engineering
• Electrochemistry
• Biosensors

Context:

• Subcutaneous glucose monitoring is crucial for diabetes management.
• Enzyme electrodes offer a promising approach for continuous glucose sensing.
• In vivo validation is essential for developing reliable glucose sensors.

Purpose:

• To introduce and evaluate an enzyme electrode for amperometric measurement of intracorporal glucose concentration.
• To compare the performance of an implanted sensor (SG) with peripheral blood glucose (PG) measurements in healthy dogs.
• To assess the long-term stability and potential degradation of the sensor in vivo.

Summary:

• An enzyme electrode (glucose oxidase, platinum-silver/silver chloride) was developed for subcutaneous glucose measurement.
• Peripheral blood glucose levels were altered via infusions and compared to sensor readings in canine models.
• A regression equation (SG = 0.81 PG - 1.39) and deviation analysis (delta SG = 0.83 delta PG + 0.22) were established.
• The sensor exhibited a 10% sensitivity loss after approximately 7.5 hours of implantation.

Impact:

• Provides insights into the in vivo performance of enzyme electrodes for glucose monitoring.
• Highlights the need for improved biocompatibility, reduced degradation, and effective sterilization for sensor development.
• Informs future research directions for creating more stable and accurate continuous glucose monitoring systems.