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Updated: May 20, 2026
Chromosome Preparation From Cultured Cells
Published on: January 28, 2014
[Cytogenetic analysis of childhood acute lymphoblastic leukemia]
Qing Liu1, Hui Jiang, Heng-juan Sun
1Department of Hematology, Children's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai, China.
Objective:
To characterize the genetic aberrations in pediatric acute lymphoblastic leukemia (ALL).
Methods:
Ninety ALL cases were enrolled in the study from January 2009 to November 2011. Chromosome banding analysis and fluorescence in situ hybridization (FISH) were used to detect genetic aberrations.
Results:
(1) Chromosome analysis: 35 (53.0%) of 66 cases who had metaphase were abnormal, and 24 cases had no metaphase. (2) FISH analysis: among the 31 cases who had normal karyotypes and 24 who had no metaphase detected by chromosome banding technique, 7 (22.6%) and 14 (58.3%) cases were abnormal detected by FISH, respectively. There were no statistically significant differences compared with chromosome analysis (P = 0.655). Among these 55 ALL cases TEL/AML1, bcr-abl and MLL fusion genes were observed in 16 (29.1%), 3(5.5%) and 2(3.6%) cases, respectively. (3) Cytogenetic aberration was observed in 56 of total 90 ALL cases (62.2%).
Conclusions:
Cytogenetic changes are common in childhood ALL. Conventional cytogenetic study could reliably detected chromosomal abnormalities for ALL with assessable metaphase. FISH should be used as a complementary method for ALL patients who have poor chromosomal morphology or no metaphase cells, and combination of both methods can improve the detection rate of genetic abnormalities in childhood leukemia.
