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Updated: May 20, 2026
Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays
Published on: September 26, 2011
Guidelines for the quantification of HIV and HCV in small volume whole blood samples
Tony Bruns1, Katrin Steinmetzer
1Division of Gastroenterology, Hepatology, and Infectious Diseases, Department of Internal Medicine II, Jena University Hospital, Jena, Germany. T.Bruns@bham.ac.uk
Abstract:
The quantification of Hepatitis C Virus (HCV) and Human Immunodeficiency Virus (HIV) in whole blood provides several advantages over the quantification in plasma samples. The application of small samples of capillary blood allows for application in point-of-care diagnostic testing methods.Here we describe two protocols of extracting viral RNA from small samples of whole blood by hybridization to biotinylated LNA-modified 2'-O-Methyl-RNA or to biotinylated DNA, indirect capturing to streptavidin-coated beads, and subsequent quantification by one-step non-nested qRT-PCR. Further, we provide some general guidelines on extraction and quantification of HIV and HCV in small volume whole blood samples.
