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Protocol for the use of enzyme-linked hybridization assays for genital ulcer disease

Seweryn Bialasiewicz1, Ian M Mackay, David M Whiley

  • 1Sir Albert Sakzewski Virus Research Centre, Herston, QLD, Australia. Seweryn@uq.edu.au

Methods in Molecular Biology (Clifton, N.J.)
|July 12, 2012
PubMed

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In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.