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Simultaneous Detection of Different Antibody Classes in a Multiplexed Serological Test
Published on: July 14, 2023
Serologic reactivity to the emerging pathogen Granulibacter bethesdensis
David E Greenberg1, Adam R Shoffner, Kimberly R Marshall-Batty
1Laboratory of Clinical Infectious Diseases, Bethesda, MD, USA. david.greenberg@utsouthwestern.edu
The Journal of Infectious Diseases
|July 12, 2012
Summary
Serologic tests reveal Granulibacter bethesdensis immunoreactivity is more common in chronic granulomatous disease (CGD) patients and healthy donors than previously thought. This suggests Granulibacter infections may be underdiagnosed in both populations.
Area of Science:
- Infectious Diseases
- Microbiology
- Immunology
Background:
- Granulibacter bethesdensis, a bacterium from the Acetobacteraceae family, has been isolated from patients with chronic granulomatous disease (CGD).
- The pathogenesis, environmental sources, and infection rates of G. bethesdensis in CGD patients and the general population remain largely unknown.
Purpose of the Study:
- To investigate the prevalence of Granulibacter immunoreactivity in individuals with and without CGD.
- To identify specific antigens of G. bethesdensis recognized by the human immune system.
Main Methods:
- Antigen identification using mass spectrometry, 2D electrophoresis, and immunoaffinity chromatography.
- Assessment of Granulibacter immunoreactivity via immunoblotting and ELISA.
- Development of an ELISA assay targeting methanol dehydrogenase (MDH) for immunoglobulin G detection.
Main Results:
- Methanol dehydrogenase (MDH) and formaldehyde-activating enzyme were identified as key antigens.
- Specific immunoreactive band patterns differentiated G. bethesdensis from other Acetobacteraceae species.
- Serologic tests indicated reactivity in 45.1% of CGD patients and 24.7% of healthy donors, with evidence of seroconversion and sustained seropositivity.
Conclusions:
- Serologic evidence suggests Granulibacter immunoreactivity is more prevalent than previously suspected in both CGD patients and healthy individuals.
- The findings indicate that clinical presentations of Granulibacter infections might be underappreciated, necessitating further investigation.
