Related Experiment Video
Updated: May 20, 2026

Microfluidic Tools for Probing Fungal-Microbial Interactions at the Cellular Level
Published on: June 23, 2022
Optimal Culture Conditions for Mycelial Growth of Lignosus rhinocerus
W H Lai1, M J Siti Murni, D Fauzi
1Agro-Biotechnology Institute, Ministry of Science, Technology and Innovation, c/o Malaysian Agricultural Research and Development Institute, 43400, Serdang, Selangor, Malaysia.
Abstract:
Lignosus rhinocerus is a macrofungus that belongs to Polyporaceae and is native to tropical regions. This highly priced mushroom has been used as folk medicine to treat diseases by indigenous people. As a preliminary study to develop a culture method for edible mushrooms, the cultural characteristics of L. rhinocerus were investigated in a range of culture media under different environmental conditions. Mycelial growth of this mushroom was compared on culture media composed of various carbon and nitrogen sources in addition to C/N ratios. The optimal conditions for mycelial growth were 30℃ at pH 6 and 7. Rapid mycelial growth of L. rhinocerus was observed on glucose-peptone and yeast extract peptone dextrose media. Carbon and nitrogen sources promoting mycelial growth of L. rhinocerus were glucose and potassium nitrate, respectively. The optimum C/N ratio was approximately 10 : 1 using 2% glucose supplemented as a carbon source in the basal media.
More Related Videos
09:39Production and Testing of Moisture Behavior and Thermal Properties of Rapeseed Straw and Ganoderma resinaceum Mycelium Bio-Composites
Published on: September 5, 2025
06:17Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions
Published on: August 15, 2019
Related Concept Videos
Fungal Group Zygomycota
Microbes in Food Production
Microbes in the Production of Fermented Foods
Production of Organic Acids
Bioreactor Controls-III