Related Experiment Video

Updated: May 20, 2026

Comparing Metastatic Clear Cell Renal Cell Carcinoma Model Established in Mouse Kidney and on Chicken Chorioallantoic Membrane
05:36

Comparing Metastatic Clear Cell Renal Cell Carcinoma Model Established in Mouse Kidney and on Chicken Chorioallantoic Membrane

Published on: February 8, 2020

CCN3 (NOV) regulates proliferation, adhesion, migration and invasion in clear cell renal cell carcinoma

Shuai Liu1, Zheng Liu, Dongbin Bi

  • 1Department of Urology, Provincial Hospital Affiliated to Shandong University, Jinan 250021, P.R. China.

Oncology Letters
|July 12, 2012
PubMed

Insights

The CCN3 gene, also known as nephroblastoma overexpressed gene, is downregulated in kidney cancer. Lower CCN3 expression correlates with increased cell proliferation and invasion in clear cell renal cell carcinoma (RCC).

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • The CCN3 gene (nephroblastoma overexpressed gene) is part of the CCN family, involved in tumorigenesis.
  • Altered CCN3 expression is linked to human nephroblastoma and renal cell carcinoma (RCC), suggesting its role in kidney cancer development.

Purpose of the Study:

  • To investigate the function of CCN3 in the biology of clear cell renal cell carcinoma (RCC).

Main Methods:

  • Immunohistochemistry was used to analyze CCN3 expression in 32 pairs of RCC and normal kidney tissues.
  • The CCN3 gene was transfected into the 786-O cell line to assess its effects on cell behavior.

Main Results:

  • CCN3 expression was significantly lower in RCC tissues compared to normal kidney tissues.
  • Reduced CCN3 expression correlated inversely with the Ki67 proliferation index.
  • CCN3-expressing cells showed inhibited proliferation, increased adhesion, migration, and invasion.

Conclusions:

  • CCN3 exhibits anti-proliferative effects in clear cell RCC cells.
  • CCN3 promotes adhesion, migration, and invasion in clear cell RCC cells, indicating a complex role in tumorigenesis.

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