Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Deoxyribonucleic acid and outer membrane: binding to outer membrane involves a specific protein.

H Wolf-Watz, A Norqvist

    Journal of Bacteriology
    |October 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The virulence protein Yop5 of Yersinia pseudotuberculosis is regulated at transcriptional level by plasmid-plB1 -encoded trans-acting elements controlled by temperature and calcium.

    Molecular microbiology·2017
    Same author

    Attenuation and preserved immunogenic potential of Yersinia pseudotuberculosis mutant strains evidenced in oral pig model.

    Zoonoses and public health·2008
    Same author

    Virulence blockers as alternatives to antibiotics: type III secretion inhibitors against Gram-negative bacteria.

    Journal of internal medicine·2008
    Same author

    Small-molecule inhibitors specifically targeting type III secretion.

    Infection and immunity·2005
    Same author

    Experimental infections with wild and mutant Yersinia pseudotuberculosis strains in rabbits.

    Journal of veterinary medicine. B, Infectious diseases and veterinary public health·2003
    Same author

    Genetic organization of the Francisella plasmid pFNL10.

    Plasmid·2001
    Same journal

    The bacterial SOS response promotes the expression of the transposase encoded by IS<i>CR</i> mobile genetic elements.

    Journal of bacteriology·2026
    Same journal

    Development of a gene-editing strategy to overcome genetic intractability in <i>Lactobacillus johnsonii</i>.

    Journal of bacteriology·2026
    Same journal

    Bactofilins are essential spatial organizers of peptidoglycan insertion in the Lyme disease spirochete <i>Borrelia burgdorferi</i>.

    Journal of bacteriology·2026
    Same journal

    DNA damage-associated vesicle production in <i>Stenotrophomonas maltophilia</i> is mediated by the maltocin endolysin.

    Journal of bacteriology·2026
    Same journal

    Characterization of <i>Helicobacter pylori</i> aggregation reveals a requirement for both AlpA and AlpB.

    Journal of bacteriology·2026
    Same journal

    Cross-regulation of amino acid synthesis and anaerobic electron transfer by MetR-mediated methionine signaling.

    Journal of bacteriology·2026
    See all related articles

    Escherichia coli outer membrane binds deoxyribonucleic acid (DNA) specifically. This interaction involves a 13,000 molecular weight protein and is disrupted by chloramphenicol or rifampin treatment.

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Biochemistry

    Background:

    • The outer membrane of Gram-negative bacteria like Escherichia coli plays a crucial role in cellular integrity and interaction with the environment.
    • Understanding interactions between extracellular molecules and bacterial membranes is vital for comprehending bacterial physiology and pathogenesis.

    Purpose of the Study:

    • To investigate the specific binding of deoxyribonucleic acid (DNA) to the outer membrane of Escherichia coli.
    • To characterize the nature of this DNA-outer membrane interaction and identify potential molecular components involved.

    Main Methods:

    • Incubation of Escherichia coli cells with deoxyribonucleic acid (DNA).
    • Quantification of bound DNA to the bacterial outer membrane.
    • Treatment of DNA-bound cells with antibiotics (chloramphenicol, rifampin) to assess complex stability.

    Related Experiment Videos

  • Analysis of potential protein involvement in the binding interaction.

    • Main Results:

    • A low amount of deoxyribonucleic acid (DNA), approximately 0.4% of total DNA, was found bound to the outer membrane.
    • Treatment with chloramphenicol or rifampin led to the dissociation of the apparent DNA-outer membrane complex.
    • Evidence suggests the involvement of a specific membrane protein with a molecular weight of 13,000 in the binding process.

    Conclusions:

    • The binding of deoxyribonucleic acid (DNA) to the Escherichia coli outer membrane is a specific interaction.
    • A membrane protein of 13,000 molecular weight is implicated in mediating this specific DNA-membrane association.
    • The DNA-outer membrane complex is sensitive to treatments that affect protein synthesis or membrane integrity.