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Mycobacterial DNA Extraction using Bead Beating in Custom Buffer Followed by NGS Workflow
Published on: June 13, 2025
Development of a new DNA extraction protocol for PFGE typing of Mycobacterium tuberculosis complex
Arash Ghodousi1, Ghodousi A Arash, S Vatani
1Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Iranian Journal of Microbiology
|July 12, 2012
Abstract:
A modified pulsed-field gel electrophoresis (PFGE) protocol was developed and applied to clinical isolates of Mycobacterium tuberculosis complex to reduce the cost of using lyticase. This protocol reduces the expense of PFGE typing of Mycobacterium tuberculosis complex as it removes the use of lyticase during the spheroplast formation from these bacteria.

