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Related Experiment Videos

Exponential gradient maker using a disposable syringe.

A Domingo1

  • 1Department of Molecular, Cellular and Developmental Biology, University of Colorado 80309-0347.

Analytical Biochemistry
|August 15, 1990
PubMed
Summary
This summary is machine-generated.

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A simple syringe modification creates an easy-to-use exponential gradient maker. This reusable or disposable device is ideal for preparing solutions and gels, especially with toxic chemicals.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • Gradient makers are essential tools for preparing solutions with varying concentrations.
  • Existing gradient maker designs can be complex or expensive.
  • There is a need for simple, cost-effective gradient generation methods.

Purpose of the Study:

  • To develop a simple, low-cost, and reusable exponential gradient maker.
  • To adapt a disposable syringe for reliable gradient generation.
  • To provide a potentially disposable option for experiments involving hazardous materials.

Main Methods:

  • Modification of a standard disposable syringe by creating notches in the plunger tip.
  • Utilizing a clamp to control solution flow.

Related Experiment Videos

  • Filling the syringe with initial solution and then introducing the notched plunger.
  • Introducing the second solution and controlling outflow via the clamp and notches for mixing.

    • Main Results:

    • The modified syringe reliably produced exponential gradients.
    • The device allowed for controlled mixing of two solutions.
    • The apparatus proved to be reusable and cost-effective.

    Conclusions:

    • A simple modification transforms a disposable syringe into an effective exponential gradient maker.
    • This method offers a practical and economical approach for solution preparation, particularly for polyacrylamide gel electrophoresis.
    • The disposable nature is advantageous when working with toxic substances.