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Related Experiment Video

Updated: May 20, 2026

Extracellular Vesicle Tissue Factor Activity Assay
03:53

Extracellular Vesicle Tissue Factor Activity Assay

Published on: December 29, 2023

Tissue factor-Akt signaling triggers microvessel formation.

G Arderiu1, E Peña, R Aledo

  • 1Cardiovascular Research Center (CSIC-ICCC), Hospital de Sant Pau (UAB), IIB-Sant Pau, Barcelona CiberOBN, Instituto de Salut Carlos III, Spain.

Journal of Thrombosis and Haemostasis : JTH
|July 13, 2012
PubMed
Summary

Tissue factor (TF) directly interacts with Akt to activate Raf/ERK and Ets-1 signaling, promoting endothelial cell tube formation and microvessel development.

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Published on: February 14, 2017

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Angiogenesis Research

Background:

  • Tissue factor (TF) is critical for angiogenesis, with prior work linking TF-induced endothelial cell (EC) CCL2 release to neovascularization.
  • Understanding the precise signaling mechanisms downstream of TF in ECs is essential for targeting angiogenesis.

Purpose of the Study:

  • To elucidate the specific signaling pathways mediating TF-induced EC tube formation.
  • To investigate the interaction between TF and key signaling molecules like Akt.

Main Methods:

  • Utilized the human microvascular endothelial cell line (HMEC-1) cultured on Matrigel for three-dimensional culture.
  • Employed siRNA to inhibit endogenous TF expression and TF overexpression to study signaling effects.
  • Performed immunoprecipitation and Western blotting to confirm TF-Akt interaction.

Main Results:

  • TF inhibition reduced EC tube formation, Akt activation, and ERK1/2 phosphorylation, while increasing Raf phosphorylation at Ser(259).
  • TF overexpression enhanced tube formation and Akt activation; a direct interaction between TF and Akt was confirmed.
  • Silencing TF's effects were partially reversed by a PAR2 agonist, suggesting PAR2-independent signaling downstream of TF-Akt.

Conclusions:

  • Tissue factor (TF) forms a complex with Akt in endothelial cells.
  • This TF-Akt interaction activates the Raf/ERK pathway and Ets-1 signaling.
  • The activated signaling cascade ultimately drives microvessel formation.