Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
Clinical Significance of Antibiotic Resistance01:25

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within the One...
Staphylococcal Skin Infections01:29

Staphylococcal Skin Infections

Staphylococcus aureus is a Gram-positive coccus that resides harmlessly on the skin and mucous membranes of healthy individuals. When the skin barrier is breached, it can shift from a commensal to an opportunistic pathogen. This transition is facilitated by surface adhesins, such as clumping factor B and S. aureus surface protein G (SasG), which bind to structural proteins, including loricrin and cytokeratin, in the damaged epidermis. Protein A, another key factor, binds the Fc region of...
Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Antimicrobial Resistance Trends in Urinary Tract Infections in 6 African Countries.

JAMA network open·2026
Same author

Presumed Bilateral Retinitis Associated with JC Virus in a Patient with HIV AIDS.

Ocular immunology and inflammation·2026
Same author

Systematic surveillance of Carbapenemase-producing Enterobacterales reveals persistent spread of IMP-4 IncM2 plasmids in New Caledonia.

Communications medicine·2026
Same author

[News: Introduction of the mpox virus into the south-western Indian Ocean territories].

Virologie (Montrouge, France)·2026
Same author

Toxin gene truncation via IS1132 in non-toxigenic toxin gene-bearing (NTTB) Corynebacterium diphtheriae.

International journal of medical microbiology : IJMM·2026
Same author

Phenotypic and genotypic characterization of clinical <i>Staphylococcus lugdunensis</i> isolates: a French retrospective cohort study.

Microbiology spectrum·2026

Related Experiment Video

Updated: May 20, 2026

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations
08:03

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations

Published on: December 7, 2021

Multilocus sequence typing analysis of Staphylococcus lugdunensis implies a clonal population structure.

Benoît Chassain1, Ludovic Lemée, Jennifer Didi

  • 1University of Rouen, Rouen University Hospital, GRAM EA 2656, Rouen, France.

Journal of Clinical Microbiology
|July 13, 2012
PubMed
Summary

A new multilocus sequence typing (MLST) scheme reveals Staphylococcus lugdunensis has a clonal population structure. This tool aids in understanding the epidemiology and phylogeny of this virulent coagulase-negative Staphylococcus.

More Related Videos

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)
08:51

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)

Published on: November 4, 2016

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus
09:03

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Published on: September 5, 2013

Related Experiment Videos

Last Updated: May 20, 2026

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations
08:03

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations

Published on: December 7, 2021

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)
08:51

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)

Published on: November 4, 2016

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus
09:03

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Published on: September 5, 2013

Area of Science:

  • Microbiology
  • Genetics
  • Epidemiology

Background:

  • Staphylococcus lugdunensis is a significant pathogen within the coagulase-negative Staphylococcus group.
  • Understanding its genetic diversity and population structure is crucial for clinical and epidemiological studies.

Purpose of the Study:

  • To develop and apply a multilocus sequence typing (MLST) scheme for S. lugdunensis.
  • To investigate the genetic relationships and population structure of S. lugdunensis isolates.

Main Methods:

  • DNA sequence analysis of seven housekeeping genes (aroE, dat, ddl, gmk, ldh, recA, and yqiL) from 87 isolates.
  • Development of a multilocus sequence typing (MLST) scheme.
  • Phylogenetic analysis and linkage disequilibrium analysis.

Main Results:

  • Defined 20 sequence types (STs) and five clonal complexes.
  • No correlation found between STs and geographic source or infection type (hematogenic vs. skin/soft tissue).
  • Penicillin resistance was associated with one clonal complex; glycopeptide tolerance did not form a distinct subpopulation.

Conclusions:

  • S. lugdunensis exhibits a predominantly clonal population structure, confirmed by significant linkage disequilibrium.
  • The developed MLST scheme is a valuable tool for macroepidemiological and phylogenetic investigations of S. lugdunensis.
  • This study provides insights into the evolutionary dynamics and population genetics of this important pathogen.