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Detection of genomic alterations in carcinogen-induced mouse liver tumors by DNA fingerprint analysis
O Müller1, A Alonso, A Buchmann
1German Cancer Research Center, Institute of Experimental Pathology, Heidelberg.
Abstract:
DNA fingerprint analysis was used to study structural abnormalities in the genome of mouse liver tumor cells. Liver tumors were induced in three strains of mice, namely C57BL/6J, C3H/He and B6C3F1, by a single injection of 20 micrograms/g body wt. diethylnitrosamine on day 15 after birth. DNA from liver tumors was digested with Hinfl restriction enzyme and hybridized on Southern blots with wild-type bacteriophage M13 DNA as probe. The resulting fingerprints of tumor DNA were compared with those of DNA from normal liver tissue. Genomic aberrations were detected in two out of 68 tumors analyzed, one stemming from a C57BL/6J and the other from a C3H/He mouse.
Insights
DNA fingerprinting revealed genomic aberrations in mouse liver tumor cells. This study analyzed diethylnitrosamine-induced tumors in three mouse strains, detecting abnormalities in two samples.
Area of Science:
- Genomics
- Cancer Research
- Molecular Biology
Background:
- Hepatocellular carcinoma is a significant health concern.
- Understanding the genomic alterations in liver tumors is crucial for developing effective treatments.
- Diethylnitrosamine (DEN) is a known carcinogen used to induce liver tumors in experimental models.
Purpose of the Study:
- To investigate structural abnormalities in the genome of mouse liver tumor cells.
- To identify specific genomic aberrations associated with diethylnitrosamine-induced hepatocarcinogenesis.
- To compare DNA fingerprints of tumor cells with normal liver tissue.
Main Methods:
- DNA fingerprint analysis using Hinfl restriction enzyme digestion.
- Southern blot hybridization with wild-type bacteriophage M13 DNA as a probe.
- Induction of liver tumors in C57BL/6J, C3H/He, and B6C3F1 mice using diethylnitrosamine.
Main Results:
- Genomic aberrations were detected in 2 out of 68 analyzed liver tumors.
- Abnormalities were identified in one tumor from a C57BL/6J mouse and one from a C3H/He mouse.
- DNA fingerprinting effectively differentiated tumor DNA from normal liver DNA.
Conclusions:
- DNA fingerprint analysis is a valuable tool for detecting genomic aberrations in cancer research.
- Diethylnitrosamine-induced liver tumors in mice can exhibit structural genomic abnormalities.
- Further research is warranted to characterize the specific genomic alterations and their functional implications.