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Updated: May 20, 2026

A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli
Published on: December 9, 2017
Prokaryotic Nε-lysine acetylomes and implications for new antibiotics
Longxiang Xie1, Wu Li, Jianping Xie
1Institute of Modern Biopharmaceuticals, State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, School of Life Sciences, Southwest University, Beibei, Chongqing 400715, China.
Abstract:
Protein Nε-acetylation, a post-translational modification widespread in eukaryotes and prokaryotes, has been intensively explored due to its crucial roles in multitudinous physiologic events including transcriptional regulation, metabolic regulation, etc. The particular hotspot is the relationship between acetylation and metabolic regulation. Protein acetylation major types and functions thereof, prokaryotic acetyltransferase, deacetylases, and acetylation sites of enzymes related to glycometabolism, together with the cross-talk between acetylation and other modification, such as the phosphorylation were summarized, with emphases on those from Mycobacteria.
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