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Related Experiment Video

Updated: May 20, 2026

Assessment of the Metabolic Effects of Isocaloric 2:1 Intermittent Fasting in Mice
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Assessment of the Metabolic Effects of Isocaloric 2:1 Intermittent Fasting in Mice

Published on: November 27, 2019

Caloric restriction chronically impairs metabolic programming in mice.

Henriette Kirchner1, Susanna M Hofmann, Antje Fischer-Rosinský

  • 1Department of Internal Medicine, Metabolic Diseases Institute, Division of Endocrinology, University of Cincinnati, Cincinnati, OH, USA.

Diabetes
|July 13, 2012
PubMed
Summary
This summary is machine-generated.

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Caloric restriction improves metabolism in obese mice by controlling fat mass, not just diet. However, benefits may not last without continuous restriction, especially after a history of obesity.

Area of Science:

  • Metabolic research
  • Obesity research
  • Nutritional science

Background:

  • Rising obesity rates necessitate effective therapies.
  • Caloric restriction is a proven safe intervention.
  • The role of adipose tissue versus dietary lipids in obesity disorders requires clarification.

Purpose of the Study:

  • To investigate if increased adipose tissue, rather than excess dietary lipids, drives obesity-associated disorders.
  • To assess the metabolic effects of clamping fat mass in mice on a high-fat diet.
  • To determine the long-term impact of caloric restriction on previously obese mice.

Main Methods:

  • Lean mice on a high-fat diet (HFD) had their fat mass (FM) clamped to match standard diet (SD) mice.
  • Diet-induced obese mice were food-restricted to match the FM of age-matched SD mice.

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Last Updated: May 20, 2026

Assessment of the Metabolic Effects of Isocaloric 2:1 Intermittent Fasting in Mice
08:06

Assessment of the Metabolic Effects of Isocaloric 2:1 Intermittent Fasting in Mice

Published on: November 27, 2019

Body Composition and Metabolic Caging Analysis in High Fat Fed Mice
10:28

Body Composition and Metabolic Caging Analysis in High Fat Fed Mice

Published on: May 24, 2018

Manipulation of Rhythmic Food Intake in Mice Using a Custom-Made Feeding System
07:34

Manipulation of Rhythmic Food Intake in Mice Using a Custom-Made Feeding System

Published on: December 16, 2022

  • Metabolic parameters including glucose and insulin tolerance, inflammation, and hyperphagia were measured.

    • Main Results:

    • Clamping fat mass in lean mice on HFD improved glucose and insulin tolerance compared to ad libitum HFD or SD mice.
    • These improvements correlated with reduced inflammation and better metabolism.
    • Food-restricted previously obese mice showed improved metabolic profiles but developed significant fasting-induced hyperphagia.
    • Hyperphagia led to accelerated weight regain upon HFD refeeding.

    Conclusions:

    • Obesity-associated disorders may be more closely linked to increased adipose tissue than dietary fat alone.
    • Caloric restriction on an HFD offers metabolic benefits, but these may be transient.
    • Individuals with a history of obesity may require lifelong caloric restriction to maintain metabolic benefits and prevent weight regain.