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Updated: May 20, 2026

Production of High-Titer Recombinant Newcastle Disease Virus from Allantoic Fluid
Published on: May 25, 2022
A majority of infectious Newcastle disease virus particles contain a single genome, while a minority contain multiple
Peter H Goff1, Qinshan Gao, Peter Palese
1Department of Microbiology, Mount Sinai School of Medicine, New York, New York, USA.
Abstract:
Paramyxoviruses produce pleiomorphic particles containing variable amounts of genetic material that correlate with virion diameter by electron microscopy. However, the infectious nature of these particles is unknown, and functional genomes are indistinguishable from defective RNA. A quantitative approach to paramyxovirus packaging revealed a majority of infectious Newcastle disease viruses contain one functional genome. Virions encapsidating two or three genomes (approximately 25% of total) were also observed by utilizing three different recombinant viruses expressing unique fluorescent reporters.
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