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Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins
09:40

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

Published on: June 11, 2015

Complete genome sequences of two Pseudomonas aeruginosa temperate phages, MP29 and MP42, which lack the phage-host

In-Young Chung1, You-Hee Cho

  • 1Department of Pharmacy, College of Pharmacy, CHA University, Gyeonggi-do, South Korea.

Journal of Virology
|July 13, 2012
PubMed

Insights

We sequenced two Pseudomonas aeruginosa phages, MP29 and MP42. Unlike similar phages, their lysogens maintained swarming ability, indicating no phage-host CRISPR interaction.

Area of Science:

  • Microbiology
  • Virology
  • Genomics

Background:

  • Pseudomonas aeruginosa temperate phages can influence host bacterial behavior.
  • Some phages, like DMS3 and MP22, impair bacterial swarming motility through interactions with host CRISPR systems.

Purpose of the Study:

  • To determine the complete genome sequences of Pseudomonas aeruginosa phages MP29 and MP42.
  • To investigate the impact of MP29 and MP42 lysogeny on P. aeruginosa swarming motility.
  • To explore potential phage-host CRISPR interactions.

Main Methods:

  • Whole genome sequencing of phages MP29 and MP42.
  • Bacteriophage lysogen construction.
  • Swarming motility assays for lysogenic bacterial strains.

Main Results:

  • The complete genome sequences of phages MP29 and MP42 were determined.
  • Lysogens of MP29 and MP42 exhibited proficient swarming motility.
  • Genomic comparison revealed similarities to phages DMS3 and MP22, but functional differences in host interaction.

Conclusions:

  • Phages MP29 and MP42 do not impair P. aeruginosa swarming motility.
  • The absence of swarming impairment suggests that these phages do not engage in the same CRISPR-mediated host interaction as phages DMS3 and MP22.

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