Related Experiment Video

Updated: May 20, 2026

Gastric Mucosa Quantitative Polymerase Chain Reaction Analysis for Detecting Helicobacter pylori and Antibiotic Resistance
05:23

Gastric Mucosa Quantitative Polymerase Chain Reaction Analysis for Detecting Helicobacter pylori and Antibiotic Resistance

Published on: March 7, 2025

P-glycoprotein expression in Helicobacter pylori-positive patients: the influence of MDR1 C3435T polymorphism

Marhanis Omar1, Andrew Crowe, Richard Parsons

  • 1School of Pharmacy, Curtin University, PO Box U1987, Perth, WA 6845, Australia. m.omar3@postgrad.curtin.edu.au

Journal of Digestive Diseases
|July 14, 2012
PubMed
Abstract

Insights

Helicobacter pylori infection and MDR1 C3435T gene variations influence P-glycoprotein expression in the upper gut. The TT genotype showed lower P-gp levels, especially with H. pylori infection.

Area of Science:

  • Gastroenterology
  • Pharmacogenomics
  • Infectious Diseases

Background:

  • P-glycoprotein (P-gp) is a key efflux transporter in the upper gastrointestinal tract.
  • Its expression can be influenced by genetic factors and infections.
  • Understanding these influences is crucial for drug absorption and efficacy.

Purpose of the Study:

  • To investigate the impact of Helicobacter pylori (H. pylori) infection on P-gp expression.
  • To determine the role of the multidrug resistance protein 1 (MDR1) C3435T polymorphism in P-gp expression.
  • To assess the combined effect of H. pylori infection and MDR1 genotype on P-gp levels.

Main Methods:

  • Upper gastrointestinal biopsies and blood samples were collected from 76 patients.
  • P-gp expression was analyzed in antral and duodenal biopsies.
  • H. pylori infection status was confirmed using culture and PCR.
  • MDR1 C3435T polymorphism was genotyped.

Main Results:

  • H. pylori infection significantly altered P-gp expression (P=0.028).
  • The TT genotype of MDR1 C3435T showed significantly lower P-gp expression compared to the CC genotype in antral tissues (P=0.041).
  • A significant difference in P-gp expression was observed in patients with the TT genotype and H. pylori infection compared to H. pylori-negative patients (P=0.029).

Conclusions:

  • H. pylori infection is associated with altered P-gp expression in the upper gastrointestinal tract.
  • The TT genotype of the MDR1 C3435T polymorphism is linked to reduced P-gp expression in the stomach.
  • These findings highlight the interplay between infection, genetic variations, and drug transporter expression.

More Related Videos

Multi-Gene Single Nucleotide Polymorphism Detection in Gastric Cancer Based on Ion Semiconductor Sequencing Platform06:21

Multi-Gene Single Nucleotide Polymorphism Detection in Gastric Cancer Based on Ion Semiconductor Sequencing Platform

Published on: May 10, 2024

One-step Negative Chromatographic Purification of Helicobacter pylori Neutrophil-activating Protein Overexpressed in Escherichia coli in Batch Mode10:44

One-step Negative Chromatographic Purification of Helicobacter pylori Neutrophil-activating Protein Overexpressed in Escherichia coli in Batch Mode

Published on: June 18, 2016

Related Concept Videos

Pharmacogenetics of Drug Transporters: P-Glycoprotein and Solute Carrier Transporters01:16

Pharmacogenetics of Drug Transporters: P-Glycoprotein and Solute Carrier Transporters

The pharmacogenetics of drug transporters is increasingly recognized as a critical factor influencing interindividual variability in drug absorption, distribution, and elimination. These membrane-bound proteins regulate drugs' movement across cellular barriers by actively pumping them out (efflux) or facilitating their uptake (influx). Among the major transporter families, ATP-binding cassette (ABC) and solute carrier (SLC) transporters play particularly prominent roles. Genetic polymorphisms...
Gastritis II: Pathophysiology01:26

Gastritis II: Pathophysiology

The pathophysiology of gastritis begins with the colonization of the stomach lining by Helicobacter pylori (H. pylori). This bacterium spreads mainly via the oral-oral route through saliva or shared utensils, and can also be transmitted in overcrowded or unhygienic environments through contaminated water, despite its brief survival outside the body.ColonizationOnce ingested, H. pylori enters the stomach and begins colonization by navigating through the mucus layer lining the stomach wall. It...
Treating Helicobacter pylori in Peptic Ulcers: Antimicrobial Therapy01:16

Treating Helicobacter pylori in Peptic Ulcers: Antimicrobial Therapy

Helicobacter pylori, a resilient gram-negative bacterium, can thrive in the stomach's harsh, acidic environment. Infection with H. pylori leads to a cascade of events within the stomach lining. One of the critical disruptions caused by this bacterium is the interference with somatostatin production, a hormone responsible for regulating acid secretion. This interference tips the balance, escalating acid secretion and diminishing bicarbonate levels. This imbalance compromises the defensive...
Pharmacogenetic Phenotypes: Alterations in Pharmacokinetics, Drug Targets and Biologic Milieu01:29

Pharmacogenetic Phenotypes: Alterations in Pharmacokinetics, Drug Targets and Biologic Milieu

Genetic variations significantly influence drug response through pharmacokinetics, receptor interactions, and biologic milieu modifications. Pharmacokinetic alterations impact drug metabolism and clearance, affecting efficacy and toxicity. Variants in drug-metabolizing enzymes, such as CYP2C9 and CYP2C19, alter drug activation and elimination. For example, CYP2C9 loss-of-function variants require lower warfarin doses to prevent excessive bleeding, while CYP2C19 variants reduce clopidogrel...
Peptic Ulcer01:27

Peptic Ulcer

Peptic ulcers are erosive lesions of the gastric or duodenal lining, most commonly caused by Helicobacter pylori infection. This Gram-negative, helical bacterium has adapted to survive the stomach’s acidic environment by producing urease, which converts urea into ammonia and carbon dioxide. The ammonia neutralizes gastric acid in the bacterium’s immediate environment, allowing colonization of the gastric mucosa. H. pylori attaches to mucus-secreting epithelial cells, penetrates the mucus...
Pharmacogenetics of Phase II Enzymes: N-acetyltransferase, Thiopurine S-methyltransferase, UDP-glucuronosyltransferase01:27

Pharmacogenetics of Phase II Enzymes: N-acetyltransferase, Thiopurine S-methyltransferase, UDP-glucuronosyltransferase

Phase II biotransformation reactions are essential for detoxifying and eliminating xenobiotics, including many pharmaceutical compounds. These reactions typically involve conjugation, the covalent attachment of polar endogenous groups such as glucuronic acid, sulfate, methyl, or acetyl moieties to functional groups introduced during Phase I metabolism. The resulting conjugates are more water-soluble, enabling efficient renal or biliary excretion.The major classes of Phase II enzymes include...