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Related Experiment Video

Updated: May 20, 2026

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform

Published on: February 25, 2021

Cationic core-shell liponanoparticles for ocular gene delivery.

Min Jiang¹, Li Gan, Chunliu Zhu

¹Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.

|July 14, 2012

Summary

This summary is machine-generated.

A novel cationic core-shell liponanoparticle enhances ocular gene delivery by improving cellular uptake and endolysosome escape. This nanoparticle strategy shows promise for effective eye-drop gene therapy.

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Last Updated: May 20, 2026

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform

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Published on: February 25, 2021

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Spatio-Temporal In Vivo Imaging of Ocular Drug Delivery Systems using Fiberoptic Confocal Laser Microendoscopy

Spatio-Temporal In Vivo Imaging of Ocular Drug Delivery Systems using Fiberoptic Confocal Laser Microendoscopy

Published on: September 27, 2021

Area of Science:

Biomaterials Science
Ocular Drug Delivery
Gene Therapy

Background:

Ocular gene therapy faces challenges in efficient gene delivery.
Current methods often struggle with low transfection efficiency and cellular uptake.

Purpose of the Study:

To design and evaluate a cationic core-shell liponanoparticle (DLCS-NP) for enhanced ocular gene transfection.
To investigate the cellular uptake mechanisms and endolysosome escape facilitated by the nanoparticle structure.

Main Methods:

Development of DLCS-NP by encapsulating plasmid-laden chitosan nanoparticles (CS-NP) within a cationic lipid shell.
Assessment of cellular uptake efficiency compared to CS-NP and lipid-coated chitosan nanoparticles (LCS-NP).
Investigation of endocytosis pathways and endolysosome escape using EGFP as a reporter gene in vitro and in vivo (rabbit models).

Main Results:

DLCS-NP demonstrated significantly higher cellular uptake (up to 5-fold) compared to control nanoparticles.
The cationic lipid shell facilitated multiple endocytic pathways and enhanced endolysosome escape.
Increased EGFP expression was observed in DLCS-NP treated cells (3.1-3.5 fold) and in vivo (2.52 fold increase).

Conclusions:

The developed DLCS-NP exhibits superior DNA protection, cellular uptake, and endolysosome escape capabilities.
This nanoparticle system represents a promising strategy for improving ocular gene delivery via eye-drop therapy.