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Updated: May 20, 2026

MicroRNA-based Regulation of Picornavirus Tropism
Published on: February 6, 2017
Suppression of feline calicivirus replication using small interfering RNA targeted to its polymerase gene
Satoshi Taharaguchi1, Takahisa Matsuhiro, Hayato Harima
1Department of Microbiology II, School of Veterinary Medicine, Azabu University, Kanagawa, Japan. tsatoshi@azabu-u.ac.jp
Abstract:
Feline calicivirus (FCV) is a pathogenic microorganism that causes upper respiratory diseases in cats. Recently, an FCV infection with a high mortality rate has been confirmed, and there is need to develop a treatment for cases of acute infection. We evaluated whether the replication of FCV could be prevented by RNA interference. For this study, we designed an siRNA targeted to the polymerase region of the strain FCV-B isolated from a cat that died after exhibiting neurological symptoms. Cells transfected with siR-pol dose-dependently suppressed the replication of FCV-B. siR-pol suppressed its replication by suppressing the target viral RNA.
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