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Updated: May 20, 2026

Screening and Identification of Small Peptides Targeting Fibroblast Growth Factor Receptor2 using a Phage Display Peptide Library
Published on: September 30, 2019
Peptide-based targeting of the platelet-derived growth factor receptor beta
Vasileios Askoxylakis1, Annabell Marr, Annette Altmann
1Department of Radiation Oncology, University of Heidelberg and German Cancer Research Center, Heidelberg, Germany. vasileios.askoxylakis@med.uni-heidelberg.de
Purpose:
The aim of this work is to identify new ligands targeting the platelet-derived growth factor receptor beta (PDGFRβ).
Procedures:
Biopanning was carried out with a 12-amino-acid phage display library against the recombinant extracellular domain of PDGFRβ. The identified peptide PDGFR-P1 was chemically synthesized and labeled with (125)I or (131)I. In vitro studies were performed on the PDGFRβ-expressing cell lines BxPC3 and MCF7 and on PDGFRβ-transfected HEK cells in comparison to negative control wtHEK293 and CaIX-transfected HEK cells. Biodistribution experiments were performed in Balb/c nude mice, carrying subcutaneously BxPC3 tumors.
Results:
In vitro studies demonstrated a higher binding to BxPC3, MCF7, and PDGFRβ-tr-HEK cells in comparison to negative control cell lines. Binding was inhibited up to 90% by the unlabeled PDGFR-P1 peptide. Organ distribution studies revealed a higher accumulation in BxPC3 tumors than in most organs.
Conclusions:
PDGFR-P1 is a promising candidate for targeting human PDGFRβ.
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