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Gold nanoparticles for immunogold localization of antigens in plant tissues

Sadaki Yokota1

  • 1Division of Functional Morphology, Nagasaki International University, Sasebo, Nagasaki, Japan. syokota@niu.ac.jp

Methods in Molecular Biology (Clifton, N.J.)
|July 14, 2012
PubMed

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Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.