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Updated: May 20, 2026
Arabidopsis thaliana Polar Glycerolipid Profiling by Thin Layer Chromatography (TLC) Coupled with Gas-Liquid Chromatography (GLC)
Published on: March 18, 2011
A quick, optimized method for routine analysis of essential and trans-octadecenoic acids in edible fats and oils by
Luis Miguel Rodríguez-Alcalá1, María Visitación Calvo, Javier Fontecha
1Departamento de Bioactividad y Análisis de Alimentos, Instituto de Investigación en Ciencias de la Alimentación CIAL (CSIC-UAM) CEI UAM + CSIC. C/ Nicolás Cabrera, 9, Campus de la Universidad Autónoma de Madrid (UAM), 28049 Madrid, Spain.
Abstract:
A gas chromatography procedure using a 30-m capillary column (VF-23ms) has been optimized for quick analysis (run time less than 20 min). The method is suitable for routine analysis of the fatty acid composition of edible fat and oil samples (milk, fish, vegetal and synthetic origin). The results were comparable with those obtained with a 100-m-long CP-Sil 88 column (run times between 60 and more than 100 min) in the analysis of geometric and positional isomers of polyunsaturated fatty acids. The achieved resolution of compounds from 4 to 26 carbon atoms with 0-6 double bonds, as omega-3 and omega-6 fatty acids, major trans-octadecenoic isomers and conjugated linoleic acid isomers, was higher than those reported in other proposed and reference methods for similar samples using short-length columns. The response factors obtained from the fatty acid composition of reference milk fat exhibited high feasibility and the inter-assay (VF23ms versus CP-Sil 88) and intra-assays based on relative standard deviation showed good accuracy, because they were lower than 10%.
