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Updated: Jan 13, 2026

Rapid High-throughput Species Identification of Botanical Material Using Direct Analysis in Real Time High Resolution Mass Spectrometry
Published on: October 2, 2016
Comparison of two species of Notopterygium by high-performance liquid chromatography-photodiode array
Guo-Qang Liu1, Jing Dong, Hong Wang
1School of Pharmaceutical Sciences, Peking University, Beijing 100191, China. lgq19840713@yahoo.com.cn
Abstract:
Notopterygium incisum Ting ex H.T. Chang (N. incisum) and Notopterygium forbesii Boiss (N. forbesii) are two medicinal species of Qianghuo (a well-known traditional herbal medicine in China) that are widely used in clinical prescriptions for the treatment of colds and rheumatism. To compare the chemical constituents of these two plant materials, the phenolic constituents and coumarins of these two species were comprehensively and systematically analyzed by high-performance liquid chromatography-photodiode array detection-electrospray ionization tandem mass spectrometry (HPLC/DAD/ESI-MS(n)) for the first time. A total of 25 compounds (nine phenolic compounds and 16 coumarins) were detected in the methanol extracts. These compounds were separated on a C18 column and identified or tentatively characterized on the basis of their UV spectra and MS fragmentation behavior. In contrast to previous reports, we found that these two plant species possess very different coumarin patterns. O-Demethylfuropinnarin (18), phenethylferulate (19), notopterol (20), and isoimperatorin (22) were the predominant constituents of N. incisum, whereas nodakenin (6), 6-O-trans-feruloylnodakenin (12), p-hydroxypenethylanisate (16) and isoimperatorin (22) were the major constituents of N. forbesii. O-Demethylfuropinnarin (18), phenethylferulate (19) and notopterol (20) were only detected in N. incisum and can be regarded as useful taxonomic markers for differentiating these two plant species. Considering the marked differences in the main chemical constituents of N. incisum and N. forbesii, the biological activities of these two species should be further investigated and compared to ensure consistency and efficacy in the pharmaceutical applications of these materials.
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