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Updated: May 20, 2026

05:35
Quantification of Metal Leaching in Immobilized Metal Affinity Chromatography
Published on: January 17, 2020
[Using of metal-affinity chromatography for identification of alkylated rat globin]
Bioorganicheskaia Khimiia
|July 17, 2012
Abstract:
Rat globin peptides alkylated by sulfur mustard on amino-acid residues C-126, C-93 and E-27 with MH+ 1444.62 Da, 1561.66 Da, 1676.78 Da, respectively, were concentrated using metal-affinity chromatography on Cu2+. The peptides were received by trypic digestion after in vitro incubation of rat globin with 60 microM HD. Aklylated peptide with MH+ 1444.62 Da is the most sensitive biomarker, which can be concentrated from globin trypic digest, incubated with 3 microM sulfur mustard.

