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Mapping Absolute DNA Density in Cell Nuclei using Single-molecule Localization Microscopy

Mapping Absolute DNA Density in Cell Nuclei using Single-molecule Localization Microscopy

Published on: November 11, 2025

A Pascal program for computing the DNA concentration in cellular homogenates.

A Sedo¹, M Adamec

¹Tuberculosis and Respiratory Diseases Research Institute, Prague, Czechoslovakia.

Computer Methods and Programs in Biomedicine

|November 1, 1990

Summary

This summary is machine-generated.

Manual evaluation of Brunk's fluorometric DNA concentration assay results is subjective and slow. A Turbo Pascal program was developed to automate fluorometric data processing for improved efficiency.

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Last Updated: Jan 4, 2026

Mapping Absolute DNA Density in Cell Nuclei using Single-molecule Localization Microscopy

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Published on: November 11, 2025

DNA Staining Method Based on Formazan Precipitation Induced by Blue Light Exposure

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Published on: January 28, 2018

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Published on: February 17, 2011

Area of Science:

Biochemistry
Molecular Biology
Analytical Chemistry

Background:

Manual graphical methods for Brunk's fluorometric DNA concentration assay are subjective.
These manual methods are also time-consuming, hindering efficient data analysis.

Purpose of the Study:

To develop a computer-based program for processing fluorometric DNA concentration assay data.
To overcome the limitations of manual graphical evaluation methods.

Main Methods:

A short program was designed using Turbo Pascal (version 4).
The program automates the processing of fluorometric data.

Main Results:

The developed program provides an objective and faster alternative to manual methods.
Automated processing enhances the efficiency of DNA concentration analysis.

Conclusions:

Computer-assisted data processing significantly improves the evaluation of Brunk's fluorometric DNA assay.
The Turbo Pascal program offers a reliable and efficient tool for fluorometric data analysis.