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A Murine Model of Subarachnoid Hemorrhage
Published on: November 21, 2013
Temporal dynamics of microparticle elevation following subarachnoid hemorrhage
Matthew R Sanborn1, Stephen R Thom, Leif-Erik Bohman
1Department of Neurosurgery, Institute for Environmental Medicine, Perelman School of Medicine at the Universityof Pennsylvania, Philadelphia, Pennsylvania 19104, USA. Matthew.Sanborn@uphs.upenn.edu
Journal of Neurosurgery
|July 17, 2012
Summary
Microparticle (MP) levels increase after subarachnoid hemorrhage (SAH). TF- and endothelial-associated MPs, linked to thrombosis and endothelial dysfunction, predict infarction risk early post-SAH.
Area of Science:
- Neuroscience
- Cardiovascular Biology
- Immunology
Background:
- Microparticles (MPs) are cell membrane fragments implicated in thrombosis, inflammation, and endothelial dysfunction.
- Their role in subarachnoid hemorrhage (SAH)-induced complications like cerebral infarction and delayed cerebral ischemia (DCI) is not well understood.
Purpose of the Study:
- To quantify MP elevations in SAH patients.
- To determine the temporal dynamics of MP elevations.
- To analyze the correlation between MP levels and DCI-related infarction.
Main Methods:
- Peripheral blood samples collected from 22 SAH patients on multiple days post-hemorrhage.
- Plasma MPs quantified using flow cytometry after labeling with Annexin V and specific cell markers (e.g., CD142 for tissue factor, CD146 for endothelial cells).
- Clinical data, including 14-day infarction on MRI and 3-month GOS-E scores, were correlated with MP levels.
Main Results:
- All MP subtypes were elevated in SAH patients compared to controls.
- Erythrocyte-, neutrophil-, and vWF-associated MPs peaked early and declined rapidly.
- Tissue factor (TF)- and endothelial cell-associated MPs remained elevated throughout the study.
- Elevated TF- and endothelial-derived MPs at Day 1 post-SAH correlated negatively with Day 14 infarction risk.
Conclusions:
- SAH is associated with elevated levels of various MP subtypes, with distinct temporal profiles.
- MPs linked to thrombosis (TF-associated) and endothelial dysfunction (endothelial cell-associated) showed sustained elevation.
- These specific MPs are significant negative predictors of radiographic infarction at 14 days post-SAH, with predictive value as early as Day 1.
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