Temporal dynamics of microparticle elevation following subarachnoid hemorrhage

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Updated: May 20, 2026

A Murine Model of Subarachnoid Hemorrhage
07:40

A Murine Model of Subarachnoid Hemorrhage

Published on: November 21, 2013

Matthew R Sanborn1, Stephen R Thom, Leif-Erik Bohman

  • 1Department of Neurosurgery, Institute for Environmental Medicine, Perelman School of Medicine at the Universityof Pennsylvania, Philadelphia, Pennsylvania 19104, USA. Matthew.Sanborn@uphs.upenn.edu

Journal of Neurosurgery
|July 17, 2012
PubMed
Abstract

Insights

Microparticle (MP) levels increase after subarachnoid hemorrhage (SAH). TF- and endothelial-associated MPs, linked to thrombosis and endothelial dysfunction, predict infarction risk early post-SAH.

Area of Science:

  • Neuroscience
  • Cardiovascular Biology
  • Immunology

Background:

  • Microparticles (MPs) are cell membrane fragments implicated in thrombosis, inflammation, and endothelial dysfunction.
  • Their role in subarachnoid hemorrhage (SAH)-induced complications like cerebral infarction and delayed cerebral ischemia (DCI) is not well understood.

Purpose of the Study:

  • To quantify MP elevations in SAH patients.
  • To determine the temporal dynamics of MP elevations.
  • To analyze the correlation between MP levels and DCI-related infarction.

Main Methods:

  • Peripheral blood samples collected from 22 SAH patients on multiple days post-hemorrhage.
  • Plasma MPs quantified using flow cytometry after labeling with Annexin V and specific cell markers (e.g., CD142 for tissue factor, CD146 for endothelial cells).
  • Clinical data, including 14-day infarction on MRI and 3-month GOS-E scores, were correlated with MP levels.

Main Results:

  • All MP subtypes were elevated in SAH patients compared to controls.
  • Erythrocyte-, neutrophil-, and vWF-associated MPs peaked early and declined rapidly.
  • Tissue factor (TF)- and endothelial cell-associated MPs remained elevated throughout the study.
  • Elevated TF- and endothelial-derived MPs at Day 1 post-SAH correlated negatively with Day 14 infarction risk.

Conclusions:

  • SAH is associated with elevated levels of various MP subtypes, with distinct temporal profiles.
  • MPs linked to thrombosis (TF-associated) and endothelial dysfunction (endothelial cell-associated) showed sustained elevation.
  • These specific MPs are significant negative predictors of radiographic infarction at 14 days post-SAH, with predictive value as early as Day 1.

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