Estradiol benzoate decreases the blood flow through the tibia of female rats

Effect of estradiol benzoate (EB, Agofollin Depot Spofa, CSSR, 1 mg s.c. once a week for 4 weeks) on local blood flow through the tibia of intact and oophorectomized (OOX) female rats was studied using the 85Sr-microsphere method. In experiment A, the 85Sr-microsphere uptake and blood flow was increased 4 weeks after OOX and significantly decreased after EB in both the intact and OOX animals. In experiment B (intact female rats), the decrease after EB was confirmed both in microsphere uptake and tibial blood flow (48.8% and 57.1% respectively). No changes in the cardiac output and the marked fall of the uptake of labelled microspheres suggest a local circulatory reaction.