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Protein-protein Interfaces

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...

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Updated: May 20, 2026

Synthesis of an Intein-mediated Artificial Protein Hydrogel

Synthesis of an Intein-mediated Artificial Protein Hydrogel

Published on: January 27, 2014

Artificial proteins from combinatorial approaches.

Agathe Urvoas¹, Marie Valerio-Lepiniec, Philippe Minard

¹Institut de Biochimie Moléculaire et Cellulaire (IBBMC), Université Paris-Sud, CNRS, UMR 8619, Orsay, F-91405 Orsay, France.

Trends in Biotechnology

|July 17, 2012

Summary

This summary is machine-generated.

Scientists explore creating artificial proteins using experimental methods like combinatorial and directed evolution. These techniques help understand natural evolution and develop novel, functional proteins from simpler fragments.

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Protein WISDOM: A Workbench for In silico De novo Design of BioMolecules

Protein WISDOM: A Workbench for In silico De novo Design of BioMolecules

Published on: July 25, 2013

Area of Science:

Protein engineering
Synthetic biology
Biochemistry

Background:

Natural protein evolution involves constraints and mechanisms like fragment coalescence.
Understanding these processes is key to designing novel protein structures and functions.

Purpose of the Study:

To review experimental approaches for creating artificial proteins.
To explore how these methods can mimic natural evolution and yield useful proteins.

Main Methods:

Combinatorial evolution to explore protein sequence space.
Directed evolution to select for desired protein structures and activities.
Modular assembly and binary patterning for efficient protein innovation.

Main Results:

Demonstration of experimental reproduction of natural protein emergence processes.
Creation of artificial proteins from idealized fragments.
Identification of direct applications for newly designed proteins.

Conclusions:

Experimental evolution methods are powerful tools for creating artificial proteins.
These approaches offer insights into evolutionary constraints and facilitate protein design.
Artificial proteins derived from fragments show significant potential for practical applications.