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Polymer adhesive surface as flexible generic platform for multiplexed assays biochip production
Céline A Mandon1, Ophélie I Berthuy, Benjamin P Corgier
1Laboratoire de Génie Enzymatique, Membranes Biomimétiques et Assemblages Supramoléculaires, Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, Université Claude Bernard Lyon 1-University of Lyon-CNRS 5246 ICBMS, Bât. CPE-43 Bd du 11 Nov.1918, 69622 Villeurbanne, France.
A novel adhesive surface microarray platform enables high-density well plates for diverse applications. This robust technology shows promise for diagnostics, including liver disease detection directly from patient serum.
Area of Science:
- Biotechnology
- Materials Science
- Analytical Chemistry
Background:
- Microarray technology is crucial for high-throughput biological analysis.
- Existing methods face limitations in achieving high spot densities within microplate formats.
- Development of novel fabrication techniques is needed to enhance microarray capabilities.
Purpose of the Study:
- To introduce and evaluate a new microarray concept utilizing an adhesive surface.
- To demonstrate the fabrication of high-density 384 and 1536-well plates.
- To explore the application potential of this platform in diagnostics and biological assays.
Main Methods:
- Fabrication of microarrays on a flat adhesive surface.
- Assembly of microarrays with bottomless 384 and 1536-well plates.
- Application testing including oligonucleotide detection, cell culture, and immunoassays.
Main Results:
- Successful production of 384-well plates with 100 spots/well and 1536-well plates with 25 spots/well.
- Demonstrated utility in detecting liver cancer marker alpha-fetoprotein and Hepatitis C Virus in patient serum.
- Achieved robust performance directly in crude patient serum, indicating platform versatility.
Conclusions:
- The adhesive surface microarray concept offers a straightforward method for creating high-density well plates.
- The platform is versatile and applicable to various biological detection and diagnostic assays.
- This technology provides a robust and generic solution for sensitive biomarker detection in clinical samples.
