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Related Experiment Video

Updated: May 20, 2026

Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells
11:50

Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells

Published on: March 18, 2019

A novel method for banking dental pulp stem cells.

Silvia Gioventù1, Gabriella Andriolo, Ferruccio Bonino

  • 1Department of Surgical Sciences, Reconstruction and Diagnostics, University of Milan, Faculty of Dentistry, IRCCS Fondazione Ca' Granda Ospedale Maggiore Policlinico, via Francesco Sforza 35, 20122 Milano, Italy.

Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis
|July 17, 2012
PubMed
Summary
This summary is machine-generated.

Laser piercing whole teeth enables cryopreservation of dental pulp stem cells (DPSC). This method preserves DPSC viability and proliferation, crucial for future regenerative medicine applications and efficient tooth banking.

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Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
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Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Published on: November 24, 2012

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Last Updated: May 20, 2026

Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells
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14:52

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Published on: November 24, 2012

Area of Science:

  • Dental stem cell research
  • Regenerative medicine
  • Cryobiology

Background:

  • Dental pulp stem cells (DPSC) are adult stem cells with high potential for regenerative medicine.
  • Current tooth banking methods require cell purification, increasing costs and workload.
  • A novel method for whole tooth cryopreservation is needed to simplify banking and preserve cell viability.

Purpose of the Study:

  • To develop and evaluate a new method for cryopreserving dental pulp stem cells (DPSC) within whole human teeth.
  • To assess the viability and proliferation of DPSC after whole tooth cryopreservation using laser piercing.
  • To compare the efficacy of laser-assisted cryopreservation with conventional methods for tooth banking.

Main Methods:

  • Human deciduous whole teeth were cryopreserved at -80°C after creating micro-channels using Nd:YAG laser piercing.
  • Dental pulp stem cells (DPSC) were isolated, expanded, and characterized post-thawing.
  • Cell viability, proliferation, morphology, and immunophenotype were assessed and compared to controls.

Main Results:

  • DPSC isolated from laser-pierced cryopreserved teeth exhibited similar morphology, immunophenotype, viability, and proliferation rates to fresh DPSC.
  • Teeth cryopreserved without laser piercing showed a significant loss in DPSC viability and proliferation.
  • Laser piercing facilitated cryoprotectant penetration, ensuring cell preservation.

Conclusions:

  • Laser piercing is an effective technique for cryopreserving dental pulp stem cells (DPSC) within whole teeth.
  • This method significantly improves DPSC viability and proliferation compared to cryopreservation without laser assistance.
  • The developed method supports efficient whole tooth banking for future regenerative medicine applications.