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Related Experiment Video

Updated: May 20, 2026

Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells
14:23

Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells

Published on: April 16, 2012

T regulatory cell separation for clinical application.

Mauro Di Ianni1, Beatrice Del Papa, Tiziana Zei

  • 1Hematology and Clinical Immunology Section, Department of Clinical and Experimental Medicine, University of Perugia, Italy. mauro.diianni@cc.univaq.it

Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis
|July 17, 2012
PubMed
Summary

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Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

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This summary is machine-generated.

This study details a method for isolating T regulatory cells (Tregs) for use in stem cell transplants. The isolated Tregs effectively reduced graft-versus-host disease without harming general immunity.

Area of Science:

  • Immunology
  • Cell Biology
  • Transplantation Science

Background:

  • T regulatory cells (Tregs) play a crucial role in immune system regulation.
  • Graft-versus-host disease (GvHD) is a significant complication following allogeneic hematopoietic stem cell transplantation (HSCT).
  • Effective methods for isolating functional Tregs are needed to mitigate GvHD.

Purpose of the Study:

  • To develop and validate a robust method for isolating T regulatory cells (Tregs) from standard leukapheresis products.
  • To assess the purity, yield, and suppressive function of the isolated Tregs.
  • To evaluate the potential application of these isolated Tregs in allogeneic HSCT for GvHD prevention.

Main Methods:

  • Tregs were isolated using a combination of double-negative selection (anti-CD8, anti-CD19) and positive selection (anti-CD25).

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  • Seventy-two procedures were performed, with cell recovery and purity assessed via flow cytometry (CD4, CD25, CD25hi, FoxP3, CD127).
  • Inhibition assays were conducted to measure the functional suppressive capacity of the isolated Tregs.

    • Main Results:

    • A median of 263×10(6) cells were recovered per procedure.
    • Isolated cell populations exhibited high purity, with a mean of 94.5% CD4+/CD25+ cells (36.5% CD4+/CD25+hi), 79.8% FoxP3+ cells, and 12.5% CD127+ cells.
    • The isolated Tregs demonstrated significant immunosuppressive function, with a mean inhibition rate of 67%.
    • The method yielded functional Tregs suitable for allogeneic HSCT.

    Conclusions:

    • The described isolation method efficiently yields highly pure and functional T regulatory cells (Tregs).
    • These Tregs hold promise for reducing the incidence and severity of GvHD in allogeneic HSCT.
    • The approach avoids bystander inhibition of general immunity, preserving essential immune functions.