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A small molecule two-photon probe for hydrogen sulfide in live tissues
Sajal Kumar Das1, Chang Su Lim, Sun Young Yang
1Department of Chemistry, Korea University, 1-Anamdong, Seoul, 136-701, Korea.
Chemical Communications (Cambridge, England)
|July 17, 2012
Summary
A novel two-photon probe, FS1, significantly enhances fluorescence by 21-fold in response to hydrogen sulfide (H2S). This probe enables selective detection of H2S within deep rat brain tissue using advanced microscopy techniques.
Area of Science:
- Chemical Biology
- Neuroscience
- Optical Imaging
Background:
- Hydrogen sulfide (H2S) is a crucial signaling molecule in the brain.
- Accurate detection of H2S in biological tissues is challenging.
- Developing specific probes for H2S is vital for understanding its physiological roles.
Purpose of the Study:
- To develop and characterize a novel two-photon fluorescent probe for H2S detection.
- To evaluate the probe's sensitivity and selectivity for H2S.
- To demonstrate the probe's utility in biological samples at physiologically relevant depths.
Main Methods:
- Synthesis and characterization of the two-photon probe FS1.
- In vitro fluorescence measurements to assess response to H2S.
- Two-photon microscopy imaging in rat hippocampal slices.
Main Results:
- The FS1 probe exhibited a 21-fold enhancement in two-photon excited fluorescence upon exposure to H2S.
- FS1 demonstrated high selectivity for H2S detection.
- Successful selective detection of H2S was achieved in rat hippocampal slices at depths of 90-190 μm.
Conclusions:
- The FS1 probe is a sensitive and selective tool for H2S detection.
- Two-photon microscopy with FS1 allows for deep-tissue imaging of H2S in neural environments.
- This probe facilitates further investigation into the biological functions of H2S in the brain.
