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Related Experiment Video

Updated: May 20, 2026

Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development

Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development

Published on: August 10, 2015

Upcyte® microvascular endothelial cells repopulate decellularized scaffold.

Katharina Scheller¹, Iris Dally, Nadja Hartmann

¹Medicyte GmbH, Heidelberg, Germany. k.scheller@medicyte.com

Tissue Engineering. Part C, Methods

|July 18, 2012

Summary

This summary is machine-generated.

Researchers developed a novel vascularization model using upcyte microvascular endothelial cells (mvECs) and a biological vascularized scaffold (BioVaSc). This approach overcomes limitations in cell supply and differentiation, promising improved tissue engineering and organoid development.

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Last Updated: May 20, 2026

Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development

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Published on: August 10, 2015

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Comparable Decellularization of Fetal and Adult Cardiac Tissue Explants as 3D-like Platforms for In Vitro Studies

Comparable Decellularization of Fetal and Adult Cardiac Tissue Explants as 3D-like Platforms for In Vitro Studies

Published on: March 21, 2019

Area of Science:

Tissue Engineering
Biotechnology
Regenerative Medicine

Background:

Poor blood supply is a major hurdle in tissue engineering, limiting cell survival and function.
Existing microvascular endothelial cell (mvEC) sources are limited and prone to dedifferentiation.
A novel technology enables the generation of large batches of quasi-primary cells (upcyte mvECs) that maintain differentiated functionality.

Purpose of the Study:

To develop an in vitro vascularization model using upcyte mvECs and a biological vascularized scaffold (BioVaSc).
To assess the proliferation, differentiation, and functionality of upcyte mvECs within the BioVaSc model.
To evaluate the potential of this combined approach for creating vascularized bioreactor models.

Main Methods:

A decellularized porcine small bowel segment (BioVaSc) was reseeded with upcyte mvECs.
Upcyte mvECs were characterized for endothelial markers (vWF, CD31, eNOS) and functionality (tube formation, migration, LDL uptake) up to population doubling 27 (PD27).
Upcyte mvECs were cultured within the BioVaSc in a custom bioreactor for 14 days and assessed for cell survival and marker expression.

Main Results:

Upcyte mvECs proliferated for an additional 15 population doublings compared to primary cells.
Upcyte mvECs retained key endothelial characteristics and biological functions up to PD27.
Cells successfully repopulated the BioVaSc scaffold and maintained endothelial marker expression in a bioreactor for 14 days.

Conclusions:

The combination of upcyte mvECs and the BioVaSc scaffold represents a promising strategy for in vitro vascularization.
This approach addresses limitations in endothelial cell supply and maintenance of function.
The developed model holds potential for creating more physiologically relevant vascularized bioreactor systems for applications like liver tissue engineering.