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Detection of hippurate hydrolysis by Legionella spp. by using a rapid high-performance liquid chromatography method
D Marmet1, N Bornstein, J Fleurette
1Laboratoire National de la Santé, Centre National de Référence des Légionelloses, Faculté de Médecine, Lyon, France.
Journal of Clinical Microbiology
|December 1, 1990
Abstract:
A rapid high-performance liquid chromatography method was developed to determine hippurate hydrolysis by Legionella spp. Benzoic acid, an end product of enzymatic activity, was directly detected by high-performance liquid chromatography after 1 and 24 h of incubation in 1% sodium hippurate. Because of its sensitivity, this procedure offers more precise identification of some Legionella spp.